Twenty one units of platelet-rich plasma(PRP) were prepared from healthy volunteer blood donors, and each unit of the PRP was divieded into two aliquots by using transfer bags.
Using SEPACELLTM leukocyte-removal filters, each one aliquot of the PRO was filtered immediately after preparation, and the other aliquot was filtered after a 48-hour storage at a room temperature with continuous agitation. Before and after
filtration,
platelet numbers and two platelet activation markers, CD62 and CD63, were measured using hematologic autoanalyzer and flow cytometry, respectively.
The results were as follows:
1. The platelet numbers in the PRP were reduced after filtration.
2. On the point of the preparation of PRP, the mean percentage of CD63-positive platelets (32.86±11.35%) was higher than that of CD62-positive platelets(14.63±11.22%).
3. When filtered immediately after preparation of PRP, the CD62-positive platelets were significantly reduced (13.23±10.43%), however, CD63positive platelets were not significantly reduced(29.83±11.05%).
4. After 48-hour storage, both two activation markers were increased, and the markers were significantly higher in the PRPs stored after filtration than in those stored without filtration.
In conclusion CD63 would be a more sensitive platelet activation marker than CD62, and the platelets expressing CD62 seemed to be removed more than those expressing CD63 during filtration.
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